What We Do

The UC Irvine Transgenic Mouse Facility (TMF) core facility provides services for the design, generation, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. In addition to academic clients at UCI, we support academic investigators at several other sister UC-campuses and numerous other universities throughout the USA as well as providing these services to commercial clients. The TMF's research associates have a combined 130 years of experience in generation of genetically engineered mice. Our experience can be your advantage.

For Grant Submissions & Publications

To acknowledge the TMF in your publications, copy and paste the language below:

The authors acknowledge the support of the Chao Family Comprehensive Cancer Center Transgenic Mouse Facility Shared Resource, supported by the National Cancer Institute of the National Institutes of Health under award number P30CA062203. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Summary of our Services and Current Pricing:

Services Offered

mother mouse with 5 babies (drawing)



DNA Microinjection


Mediated Genome Engineering


Targeted Transgenesis


ES Cell Injection

Embryo & Sperm

Cryopreservation & Revival



ES Cell-Mediated

Gene Targeting



The TMF Uses iLab to Provide Estimates and Billing of Services


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TMF News & Announcements

New Services

CRISPR/Cas9 based targeted transgenesis at H11 locus The Hipp11 (H11) locus is a “safe-harbor” locus close to the centromere of mouse chromosome 11. Transgenes integrated into this locus can be expressed at a more consistent level than at the similar ROSA26 safe-harbor locus on chromosome 6.  An additional advantage is increased efficiency of transgenesis at…

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Improved Services

Hyper-ovulation of egg donors To continue to address the 3R’s and to reduce the cost of our services, the TMF uses hyper-ovulation to produce oocytes and zygotes for different services. The difference between conventional superovulation and hyperovulation is the inclusion of an antibody against inhibin, which is administered along with the PMSG. During superovulation, exposure…

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Innovative Strategies

Production of sex-sorted blastocysts By mating wildtype females with male mice carrying an X-linked GFP transgene, we can produce blastocysts that can be sorted by sex (female embryos are fluorescent but male embryos are not).  When such blastocysts are injected with ES cells to make chimeric mice and the resulting pups are sexed at birth,…

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Of Interest to TMF and Cancer Center Users