What We Do
The UC Irvine Transgenic Mouse Facility (TMF) core facility provides services for the design, generation, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. In addition to academic clients at UCI, we support academic investigators at several other sister UC-campuses and numerous other universities throughout the USA as well as providing these services to commercial clients. The TMF's research associates have a combined 130 years of experience in generation of genetically engineered mice. Our experience can be your advantage.
How to acknowledge the TMF in your publications:
Summary of our Services and Current Pricing:
CRISPR / Cas9
Mediated Genome Engineering
ES Cell Injection
Embryo & Sperm
Cryopreservation & Revival
The TMF Uses iLab to Provide Estimates and Billing of Services
TMF News & Announcements
CRISPR/Cas9 based targeted transgenesis at H11 locus The Hipp11 (H11) locus is a “safe-harbor” locus close to the centromere of mouse chromosome 11. Transgenes integrated into this locus can be expressed at a more consistent level than at the similar ROSA26 safe-harbor locus on chromosome 6. An additional advantage is increased efficiency of transgenesis at…Read More
Hyper-ovulation of egg donors To continue to address the 3R’s and to reduce the cost of our services, the TMF uses hyper-ovulation to produce oocytes and zygotes for different services. The difference between conventional superovulation and hyperovulation is the inclusion of an antibody against inhibin, which is administered along with the PMSG. During superovulation, exposure…Read More
Production of sex-sorted blastocysts By mating wildtype females with male mice carrying an X-linked GFP transgene, we can produce blastocysts that can be sorted by sex (female embryos are fluorescent but male embryos are not). When such blastocysts are injected with ES cells to make chimeric mice and the resulting pups are sexed at birth,…Read More
Of Interest to TMF and Cancer Center Users
The Mouse Models of Human Cancer database (MMHCdb)
(2023) Begley et al Dis. Model Mech 16 (4): dmm050001
The use of CRISPR/Cas9-based gene editing strategies to explore cancer gene function in mice. (2021) Current Opinion in Genes & Development 66:57-62.
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy. (2020) Science Advances 6, doi: 10.1126/sciadv.abc9450
Near-Infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin (2019) eLife https://doi.org/10/7554/eLife.45801.001
Is your gRNA not mediating cutting ? Maybe inclusion of a 'TT' or 'GCC' sequence in the seed domain is the reason ? (2019) Cell Reports 26, p1098
Nucleosomes inhibit target cleavage by CRISPR-CAS9 in vivo - a possible reason why targeting efficiency varies from locus to locus. (2018) PNAS 38, p9351.
Transgenic Mouse Models in Cancer Research - a review of methods to generate mouse models of human cancer and examples of their use. (2018) Frontiers in Oncology 8:268
The Mice that Grow Human Tumours - a brief news feature on utility of PDX mouse models (2018) Nature 560, p156
NCG - NOD CRISPR prkdc Il2r Gamma triple immunodeficient Mouse Model - A new mouse model for engraftment of human normal and tumor tissues.
Genetically engineered mouse models in oncology research and cancer medicine. (2017) EMBO Molecular Medicine 9, p137.