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CRISPR/Cas9 based targeted transgenesis at H11 locus The Hipp11 (H11) locus is a “safe-harbor” locus close to the centromere of mouse chromosome 11. Transgenes integrated into this locus can be expressed at a more consistent level than at the similar ROSA26 safe-harbor locus on chromosome 6. An additional advantage is increased efficiency of transgenesis at…
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Hyper-ovulation of egg donors To continue to address the 3R’s and to reduce the cost of our services, the TMF uses hyper-ovulation to produce oocytes and zygotes for different services. The difference between conventional superovulation and hyperovulation is the inclusion of an antibody against inhibin, which is administered along with the PMSG. During superovulation, exposure…
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Production of sex-sorted blastocysts By mating wildtype females with male mice carrying an X-linked GFP transgene, we can produce blastocysts that can be sorted by sex (female embryos are fluorescent but male embryos are not). When such blastocysts are injected with ES cells to make chimeric mice and the resulting pups are sexed at birth,…
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